ABCD1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ABCD1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1H8E5

Kat-Nr. : 60153-1-Ig

Synonyme

1H8E5, ABCD 1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1

---

• 

  WB analysis of HeLa using 60153-1-Ig

  WB result of ABCD1 antibody (60153-1-Ig, 1:1000) with si-Control and si-ABCD1 transfected HeLa cells.

• 

  WB analysis of HeLa using 60153-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60153-1-Ig (ABCD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 60153-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCD1 antibody (60153-1-Ig, Clone: 1H8E5 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).DAPI (blue).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60153-1-Ig bindet in WB, IF/ICC, ELISA ABCD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ABCD1 fusion protein Ag12757
• Vollständiger Name: ATP-binding cassette, sub-family D (ALD), member 1
• Berechnetes Molekulargewicht: 745 aa, 83 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC025358
• Gene symbol: ABCD1
• Gene ID (NCBI): 215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Publikationen

Species	Application	Title
		EMBO Rep; Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol; Authors - Desh Raj