ABCE1 Monoklonaler Antikörper

ABCE1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B7E8

Rezensionen (1)

Kat-Nr. : 67960-1-Ig

Synonyme

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

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Galerie der Validierungsdaten

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  WB analysis using 67960-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis using 67960-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67960-1-Ig (ABCE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67960-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67960-1-Ig (ABCE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67960-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of U2OS using 67960-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ABCE1 antibody (67960-1-Ig, Clone: 1B7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, 4T1-Zellen, A431-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67960-1-Ig bindet in WB, IHC, IF, ELISA ABCE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ABCE1 fusion protein Ag28493
• Vollständiger Name: ATP-binding cassette, sub-family E (OABP), member 1
• Berechnetes Molekulargewicht: 599 aa, 67 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC016283
• Gene symbol: ABCE1
• Gene ID (NCBI): 6059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This antibody does not work in any cell extract tested. It doesn't work in PVDF or nitrocellulose, multiple dilution and different blocking buffers.

• Applications: Western Blot
• Cell Tissue Type: HeLa, HEK293T, MT4 cells