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ABCG1 Polyklonaler Antikörper
ABCG1 Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13578-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe |
Erfolgreiche IP | Maushirngewebe |
Erfolgreiche Detektion in IHC | Maus-Plazenta-Gewebe, humanes Zervixkarzinomgewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 51 publications below |
IHC | See 7 publications below |
IF | See 10 publications below |
Produktinformation
13578-1-AP bindet in WB, IHC, IF, IP, ELISA ABCG1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | ABCG1 fusion protein Ag4479 |
Vollständiger Name | ATP-binding cassette, sub-family G (WHITE), member 1 |
Berechnetes Molekulargewicht | 678 aa, 76 kDa |
Beobachtetes Molekulargewicht | 75 kDa |
GenBank-Zugangsnummer | BC029158 |
Gene symbol | ABCG1 |
Gene ID (NCBI) | 9619 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for ABCG1 antibody 13578-1-AP | Protokoll herunterladen |
IHC protocol for ABCG1 antibody 13578-1-AP | Protokoll herunterladenl |
IP protocol for ABCG1 antibody 13578-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cancer Res PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer | ||
Autophagy Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells. | ||
Br J Pharmacol Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy. | ||
J Pharm Anal Targeted delivery of rosuvastatin enhances treatment of hyperhomocysteinemia-induced atherosclerosis using macrophage membrane-coated nanoparticles | ||
Phytomedicine MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice | ||
Phytomedicine Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shiford (Verified Customer) (09-24-2023) | We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.
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