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ABCG1 Polyklonaler Antikörper

ABCG1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(58)

Kat-Nr. : 13578-1-AP

Synonyme

ABC8, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, EC:7.6.2.-, WHT1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCMaus-Plazenta-Gewebe, humanes Zervixkarzinomgewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13578-1-AP bindet in WB, IHC, IF, IP, ELISA ABCG1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ABCG1 fusion protein Ag4479
Vollständiger Name ATP-binding cassette, sub-family G (WHITE), member 1
Berechnetes Molekulargewicht 678 aa, 76 kDa
Beobachtetes Molekulargewicht 75 kDa
GenBank-ZugangsnummerBC029158
Gene symbol ABCG1
Gene ID (NCBI) 9619
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ABCG1 antibody 13578-1-APProtokoll herunterladen
IHC protocol for ABCG1 antibody 13578-1-APProtokoll herunterladenl
IP protocol for ABCG1 antibody 13578-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cancer Res

PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer

Authors - Takehiro Yamamoto
mouseWB

Autophagy

Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.

Authors - Jun Matsuda
humanWB

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
humanIF

J Pharm Anal

Targeted delivery of rosuvastatin enhances treatment of hyperhomocysteinemia-induced atherosclerosis using macrophage membrane-coated nanoparticles

Authors - Dayue Liu
mouseWB,IF

Phytomedicine

MaiJiTong granule attenuates atherosclerosis by reducing ferroptosis via activating STAT6-mediated inhibition of DMT1 and SOCS1/p53 pathways in LDLR-/- mice

Authors - Jia Shi
mouseIHC

Phytomedicine

Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux

Authors - Jianfei Mu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Shiford (Verified Customer) (09-24-2023)

We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: Cancer cell lines from various tissues
ABCG1 Antibody Western Blot validation (1:1500 dilution) in Cancer cell lines from various tissues (Cat no:13578-1-AP)