ABLIM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ABLIM1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 15129-1-AP

Synonyme

ABLIM, abLIM 1, ABLIM1, actin binding LIM protein 1, KIAA0059, LIMAB1, LIMATIN

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15129-1-AP

  WB result of ABLIM1 antibody (15129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABLIM1 transfected HeLa cells.

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  WB analysis of HeLa using 15129-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 15129-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15129-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP (ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15129-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15129-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP (ABLIM1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 15129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 15129-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 15129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

15129-1-AP bindet in WB, IHC, IF, ELISA ABLIM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABLIM1 fusion protein Ag7258
• Vollständiger Name: actin binding LIM protein 1
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa, 105-110 kDa
• GenBank-Zugangsnummer: BC002448
• Gene symbol: ABLIM1
• Gene ID (NCBI): 3983
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Several isoforms of ABLIM1 exist due to the alternative splicing. This antibody recognized 110 kDa and 48 kDa forms of ABLIM1. The phosphorylation or binding with SUMO2 may result in higher MW (predicted MW 88 kDa).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
mouse	WB	J Cell Physiol; Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.; Authors - Haruna Narahara ; KD Validated
human	WB	Int J Biol Sci; Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.; Authors - Xin Dong ; KD Validated
	WB	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah
human	IF	medRxiv; Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies; Authors - Young Me Yoon

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (05-29-2019)

ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MDCK GII

ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)