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ACE2 Polyklonaler Antikörper

ACE2 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(146)

Kat-Nr. : 21115-1-AP

Synonyme

ACE related carboxypeptidase, ACEH, ACE-related carboxypeptidase, Angiotensin converting enzyme 2, Angiotensin-converting enzyme 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausnierengewebe, humanes Skelettmuskelgewebe
Erfolgreiche IPMausnierengewebe
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Dünndarmgewebe, Maushodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Nierengewebe, Mausnierengewebe
Erfolgreiche Detektion in IF-FroMausnierengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:1000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21115-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, CoIP, ELISA ACE2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Hund, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ACE2 fusion protein Ag15455
Vollständiger Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Berechnetes Molekulargewicht 805 aa, 92 kDa
Beobachtetes Molekulargewicht 120 kDa, 92 kDa
GenBank-ZugangsnummerBC048094
Gene symbol ACE2
Gene ID (NCBI) 59272
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ACE2 antibody 21115-1-APProtokoll herunterladen
IHC protocol for ACE2 antibody 21115-1-APProtokoll herunterladenl
IF protocol for ACE2 antibody 21115-1-APProtokoll herunterladen
IP protocol for ACE2 antibody 21115-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Genet

Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs

Authors - Antoine Rebendenne
humanIF

Cell Stem Cell

Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.

Authors - Ryan M Samuel
human,mouseIF

Cell Res

Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2.

Authors - Yunqing Gu
humanIHC

Nat Neurosci

Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19.

Authors - Jenny Meinhardt
humanWB

Nat Commun

Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology

Authors - Arthur Wickenhagen
humanWB,FC

Mol Cell

Enhanced SARS-CoV-2 entry via UPR-dependent AMPK-related kinase NUAK2

Authors - Vibhu Prasad

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Magdalena (Verified Customer) (07-18-2022)

I'm satisfied with this antibody. I used this antibody in dot-blot technique and IF. In IF I used dilution factor 1:200, seconday antibody was alexa fluor 555.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:200
FH

Ratna (Verified Customer) (01-14-2021)

Good

  • Applications: Western Blot