ACE2 Monoklonaler Antikörper

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• KD/KO Validated

ACE2 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(3)

CloneNo.

2F12A4

Kat-Nr. : CL488-66699

Synonyme

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

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  IF Staining of human kidney using CL488-66699

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.

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  IF Staining of human kidney using CL488-66699

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Nierengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

CL488-66699 bindet in IF-P ACE2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACE2 fusion protein Ag15554
• Vollständiger Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Berechnetes Molekulargewicht: 805 aa, 92 kDa
• GenBank-Zugangsnummer: BC048094
• Gene symbol: ACE2
• Gene ID (NCBI): 59272
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.

Publikationen

Species	Application	Title
	FC	Adv Mater; Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy.; Authors - Feng Xie
human	IF	J Virol; The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.; Authors - Shouwen Du
rat	IF	Mol Med Rep; Swimming training promotes angiogenesis of endothelial progenitor cells by upregulating IGF1 expression and activating the PI3K/AKT pathway in type 2 diabetic rats; Authors - Lan Li