ACLY Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ACLY Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

CloneNo.

1E10D5

Rezensionen (1)

Kat-Nr. : 67166-1-Ig

Synonyme

ATP citrate lyase, 1E10D5, ACL, ATP-citrate, ATP-citrate synthase

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• 

  WB analysis of Jurkat using 67166-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67166-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67166-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67166-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 67166-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67166-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 67166-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACLY antibody (67166-1-Ig, Clone: 1E10D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

67166-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA ACLY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACLY fusion protein Ag7709
• Vollständiger Name: ATP citrate lyase
• Berechnetes Molekulargewicht: 121 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC006195
• Gene symbol: ACLY
• Gene ID (NCBI): 47
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse,human	WB	Adv Sci (Weinh); Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells; Authors - Ling Li
mouse	WB	Phytomedicine; Melittin suppresses ovarian cancer growth by regulating SREBP1-mediated lipid metabolism; Authors - Ruixin Wu
mouse	WB	Int Immunopharmacol; ACLY inhibitor reduced Porphyromonas gingivalis-induced pyroptosis by suppressing microtubules acetylation in macrophages; Authors - Huimin Mao ; KD Validated
mouse	WB	Molecules; The Different Mechanisms of Lipid Accumulation in Hepatocytes Induced by Oleic Acid/Palmitic Acid and High-Fat Diet; Authors - Miao Zhang
human	WB	Mediators Inflamm; The Mutual Inhibition of FoxO1 and SREBP-1c Regulated the Progression of Hepatoblastoma by Regulating Fatty Acid Metabolism.; Authors - Yu Hu
human	WB,IP,IF	Cell Death Dis; ARHGEF3 regulates the stability of ACLY to promote the proliferation of lung cancer; Authors - Feifei Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Domenico (Verified Customer) (10-26-2022)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Sheep embryonic fibroblasts