ACOX3 Rekombinanter Antikörper

ACOX3 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241172G8

Kat-Nr. : 84027-4-RR

Synonyme

EC:1.3.3.6, BRCOX, BRCACox, Branched-chain acyl-CoA oxidase, ACOX 3

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  WB analysis using 84027-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using 84027-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84027-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe, MCF-7-Zellen, U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84027-4-RR bindet in WB, FC (Intra), ELISA ACOX3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACOX3 fusion protein Ag10553
• Vollständiger Name: acyl-Coenzyme A oxidase 3, pristanoyl
• Berechnetes Molekulargewicht: 624 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC017053
• Gene symbol: ACOX3
• Gene ID (NCBI): 8310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.