ACP1 Polyklonaler Antikörper

ACP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22214-1-AP

Synonyme

acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1

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  WB analysis of mouse brain using 22214-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22214-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 22214-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, 4T1-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

22214-1-AP bindet in WB, IF, IHC, ELISA ACP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACP1 fusion protein Ag17539
• Vollständiger Name: acid phosphatase 1, soluble
• Berechnetes Molekulargewicht: 158 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC007422
• Gene symbol: ACP1
• Gene ID (NCBI): 52
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human red cell acid phosphatase (ACP1) is a polymorphic enzyme closely related to cytosolic low molecular weight acid phosphatases, a protein family broadly conserved among eukaryotes. It catalyses the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. This protein has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang