ACPP Monoklonaler Antikörper

ACPP Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1G2C9

Kat-Nr. : 60176-1-Ig

Synonyme

PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3

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  WB analysis of K-562 using 60176-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60176-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 60176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 60176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 60176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 60176-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 60176-1-Ig(ACPP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human prostate cancer using 60176-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human prostate cancer using 60176-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Prostatakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

60176-1-Ig bindet in WB, IHC, IF-P, ELISA ACPP und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACPP fusion protein Ag8578
• Vollständiger Name: acid phosphatase, prostate
• Berechnetes Molekulargewicht: 418 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC007460
• Gene symbol: ACPP
• Gene ID (NCBI): 55
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

Publikationen

Species	Application	Title
human	WB	Front Endocrinol (Lausanne); Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.; Authors - Chong Wang