ACSM3 Polyklonaler Antikörper

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• KD/KO Validated

ACSM3 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10168-2-AP

Synonyme

ACSM3, Butyrate CoA ligase 3, Protein SA homolog, SA, SAH

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10168-2-AP

  WB result of ACSM3 antibody (10168-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSM3 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10168-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10168-2-AP (ACSM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10168-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10168-2-AP (ACSM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10168-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10168-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10168-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10168-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10168-2-AP (ACSM3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10168-2-AP

  Immunofluorescent analysis of HepG2 cells, using ACSM3 antibody 10168-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 10168-2-AP

  1X10^6 HepG2 cells were stained with 0.2ug ACSM3 antibody (10168-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10168-2-AP bindet in WB, IHC, IF, FC, ELISA ACSM3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSM3 fusion protein Ag0224
• Vollständiger Name: acyl-CoA synthetase medium-chain family member 3
• Berechnetes Molekulargewicht: 49 kDa, 66 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC002790
• Gene symbol: ACSM3
• Gene ID (NCBI): 6296
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.

Publikationen

Species	Application	Title
mouse,human	IHC	Cell; Organoid Models of Human and Mouse Ductal Pancreatic Cancer.; Authors - Sylvia F Boj
human	IHC	Front Cell Dev Biol; Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.; Authors - Lingyun Zhang
human	WB, IHC	Front Oncol; The Overexpression of Acyl-CoA Medium-Chain Synthetase-3 (ACSM3) Suppresses the Ovarian Cancer Progression via the Inhibition of Integrin β1/AKT Signaling Pathway.; Authors - Limei Yan
mouse		Sci Rep; Thioesterase induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation.; Authors - Giovan N Cholico
mouse	WB,IF	Eur J Cell Biol; Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy.; Authors - Paul Dowling
human	WB	Exp Ther Med; ACSM3 suppresses proliferation and induces apoptosis and cell cycle arrest in acute myeloid leukemia cells via the regulation of IGF2BP2; Authors - Xin Zheng