Alpha smooth muscle actin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Alpha smooth muscle actin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay

Konjugation

Unkonjugiert

Publikationen(1508)

Rezensionen (5)

Kat-Nr. : 14395-1-AP

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

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  WB analysis using 14395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14395-1-AP

  WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.

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  WB analysis using 14395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14395-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14395-1-AP

  IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

• 

  IHC staining of human appendicitis using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14395-1-AP (Alpha smooth muscle actin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 40x lens).

• 

  IHC staining of human colon using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of stromal tumor using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens).

• 

  IHC staining of stromal tumor using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hysteromyoma using 14395-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 14395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha smooth muscle actin antibody (14395-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 14395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 14395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of C2C12 using 14395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 14395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 14395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 14395-1-AP

  1x10^6 C2C12 cells were intracellularly stained with 0.4 ug smooth muscle actin Polyclonal antibody (14395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, A549-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, Mausherzgewebe, Mausnierengewebe, Mauslebergewebe, Ratten-Kolongewebe, Rattenherzgewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Appendizitis-Gewebe, humanes Kolongewebe, humanes Herzgewebe, humanes Uterusmyomgewebe, Maus-Kolongewebe, Stromatumorgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, H9C2-Zellen
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1500-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:1000-1:4000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:800-1:3200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 979 publications below
• IHC: See 432 publications below
• IF: See 601 publications below
• CoIP: See 1 publications below

Produktinformation

14395-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay Alpha smooth muscle actin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Hund, Kaninchen, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha smooth muscle actin fusion protein Ag5886
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 377 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC017554
• Gene symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publikationen

Species	Application	Title
human	IHC	Nat Genet; Spatially resolved transcriptomic analysis of the adult human prostate; Authors - Junyi Hu
rat	IF	Adv Mater; Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring; Authors - Huihui He
mouse,human	IF	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
mouse	WB	Cell Metab; Augmentation of scleral glycolysis promotes myopia through histone lactylation; Authors - Xiaolei Lin
mouse	IF	Mil Med Res; Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease; Authors - Guang-Hui Deng
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (02-04-2025)

high specificity and sensitivity, providing reliable and reproducible results

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:200

FH

James (Verified Customer) (09-22-2022)

Used by IF. Very well antibody.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: kidney

Alpha smooth muscle actin Antibody Immunohistochemistry validation (1 to 200 dilution) in kidney (Cat no:14395-1-AP)

FH

Wu (Verified Customer) (09-08-2022)

Used by IF. Really good signal

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: fibroblast cell

Alpha smooth muscle actin Antibody Immunofluorescence validation (1:150 dilution) in fibroblast cell (Cat no:14395-1-AP)

FH

Emma (Verified Customer) (11-29-2021)

Used by IF on FFPE prostate tissue. Tris-EDTA pH 9.0 antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

Alpha smooth muscle actin Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:14395-1-AP)

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and antigen retrieval was done in NaCit ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish ocular muscles at 3dpf