Alpha smooth muscle actin specific Polyklonaler Antikörper

Alpha smooth muscle actin specific Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(291)

Rezensionen (4)

Kat-Nr. : 55135-1-AP

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

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  WB analysis using 55135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 55135-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of rat colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

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  IHC staining of human colon cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of mouse colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of mouse liver using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse heart using 55135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 55135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Herzgewebe, humanes Leberzirrhosegewebe, humanes Leberkarzinomgewebe, Maus-Kolongewebe, Mauslebergewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 198 publications below
• IHC: See 76 publications below
• IF: See 124 publications below

Produktinformation

55135-1-AP bindet in WB, IHC, IF-P, ELISA Alpha smooth muscle actin specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_001613
• Gene symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

Publikationen

Species	Application	Title
mouse	IF	Nat Cell Biol; Ex vivo imaging reveals the spatiotemporal control of ovulation; Authors - Christopher Thomas
mouse	IF	ACS Nano; Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction; Authors - Xiaoqian Ji
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB,IHC,IF	Adv Sci (Weinh); LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease; Authors - Fuji Yang
mouse	IF	Nat Commun; Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis.; Authors - Wen Gao
human	IF	Mol Cancer; Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts.; Authors - Jing Kong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zach (Verified Customer) (09-12-2025)

The antibody works great with pancreas samples with pancreatitis, both WB and IHC-P.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas

FH

S (Verified Customer) (09-23-2024)

Excelllent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

Alpha smooth muscle actin specific Antibody Western Blot validation (1000 dilution) in A549 (Cat no:55135-1-AP)

FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: cardiac Fibroblast

Alpha smooth muscle actin specific Antibody Western Blot validation ( dilution) in cardiac Fibroblast (Cat no:55135-1-AP)

FH

S (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: LUNG FIBROBLASTS

Alpha smooth muscle actin specific Antibody Western Blot validation ( dilution) in LUNG FIBROBLASTS (Cat no:55135-1-AP)