• Featured Product
  • KD/KO Validated

Beta Actin Polyklonaler Antikörper

Beta Actin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Hund, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4703)

Kat-Nr. : 20536-1-AP

Synonyme

Actin, ACTB, bate actin, β actin, Beta-actin



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, A549-Zellen, C6-Zellen, Caco-2-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Maus-Kolongewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenhirngewebe, Rattennierengewebe, Rattenlebergewebe, Rattenmilzgewebe, RAW 264.7-Zellen, SMMC-7721-Zellen
Erfolgreiche Detektion in IHChumanes Kolongewebe, humanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:4000-1:10000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20536-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Beta Actin und zeigt Reaktivität mit Affe, human, Hund, Maus, Ratten

Getestete Reaktivität Affe, human, Hund, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hefe, Huhn, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Beta Actin fusion protein Ag14521
Vollständiger Name actin, beta
Berechnetes Molekulargewicht 375 aa, 42 kDa
Beobachtetes Molekulargewicht 42 kDa
GenBank-ZugangsnummerBC002409
Gene symbol Beta Actin
Gene ID (NCBI) 60
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Beta Actin antibody 20536-1-APProtokoll herunterladen
IHC protocol for Beta Actin antibody 20536-1-APProtokoll herunterladenl
IF protocol for Beta Actin antibody 20536-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen

Nature

Promotion of DNA end resection by BRCA1-BARD1 in homologous recombination

Authors - Sameer Salunkhe
humanWB

Cancer Cell

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy

Authors - Chen Yang
mouseWB

Nature

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Authors - Matthew B Johnson

Signal Transduct Target Ther

Identifying genetic targets in clinical subtypes of Parkinson's disease for optimizing pharmacological treatment strategies

Authors - Dewen Kong
WB

Cell

Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes.

Authors - Hannah Scheiblich

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Matthieu (Verified Customer) (09-24-2025)

Produces a clear band in WB at the expected molecular weight

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human fibroblasts
FH

Manon (Verified Customer) (09-17-2025)

Very good housekeeping antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Fibroblasts
FH

YINGJIAN (Verified Customer) (05-27-2025)

This primary antibody is effective, even at a low concentration (1:10000).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: mouse liver
Beta Actin Antibody Western Blot validation (1:10000 dilution) in mouse liver (Cat no:20536-1-AP)
FH

Julia (Verified Customer) (06-20-2023)

Our lab has been using Proteintech's beta actin polyclonal antibody for years. We use this antibody in all our western blot experiments for normalization. This antibody works consistently at a 1:1000 dilution for human and mouse retina and for HRECs. We have always gotten a beautiful result, with nice thick and specific bands. The only downside is that this antibody is hard to strip. Even after stripping, the membrane tends to have residual beta actin bands. Even so, we would highly recommend this product for routine western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse retina, human retina, human retinal endothelial cell line
Beta Actin Antibody Western Blot validation (1:1000 dilution) in mouse retina, human retina, human retinal endothelial cell line (Cat no:20536-1-AP)
FH

Chun (Verified Customer) (06-19-2022)

This antibody worked very well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells and HEK293 cells
FH

Aaron (Verified Customer) (12-08-2020)

nice bands achieved

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Rat epidermal keratinocytes
FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for basal heart tissues.Good for internal control for heart diseases.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

SCOTT (Verified Customer) (10-11-2019)

Used 1/1000 overnight 4 C. overnightHuman Grey matter cortex post mortem material

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human Grey matter cortex post mortem
Beta Actin Antibody Western Blot, validation (1/1000 dilution) in Human Grey matter cortex post mortem (Cat no:20536-1-AP)
FH

Dan (Verified Customer) (08-19-2019)

The antibody works

  • Applications: Western Blot, Immunohistochemistry,
FH

Kishor (Verified Customer) (01-30-2019)

Working well for Western Blotting (1:5000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
Beta Actin Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:20536-1-AP)