Beta Actin Monoklonaler Antikörper

Beta Actin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Pflanze, Ratte, Zebrafisch und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3285)

CloneNo.

7D2C10

Kat-Nr. : 60008-1-Ig

Synonyme

ACTB, Actin, bate actin, β actin, Actin cytoplasmic 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMultizellen/-gewebe, A549-Zellen, Arabidopsis-Gewebe (aus der gesamten Pflanze), HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, Reisgewebe (aus der gesamten Pflanze)
Erfolgreiche Detektion in IHCMausherzgewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PRattenhirngewebe
Erfolgreiche Detektion in IF/ICCMDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)-PIF-P : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60008-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA Beta Actin und zeigt Reaktivität mit human, Maus, Pflanze, Ratten, Zebrafisch

Getestete Reaktivität human, Maus, Pflanze, Ratte, Zebrafisch
In Publikationen genannte Reaktivitäthuman, arabidopsis, Hefe, Huhn, Ratte, Ziege
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen Beta Actin fusion protein Ag0297
Vollständiger Name actin, beta
Berechnetes Molekulargewicht 375 aa, 42 kDa
Beobachtetes Molekulargewicht 42 kDa
GenBank-ZugangsnummerBC002409
Gene symbol Beta Actin
Gene ID (NCBI) 60
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Caprylsäure/Ammoniumsulfat-Präzipitation
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Beta Actin antibody 60008-1-IgProtokoll herunterladen
IHC protocol for Beta Actin antibody 60008-1-IgProtokoll herunterladenl
IF protocol for Beta Actin antibody 60008-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,CoIP

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
WB

Nature

A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
mouseWB

Immunity

Microglial lipid phosphatase SHIP1 limits complement-mediated synaptic pruning in the healthy developing hippocampus

Authors - Alessandro Matera
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
humanWB

Cell Metab

Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer

Authors - Hannah N Bell

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Reyes (Verified Customer) (02-10-2025)

Beta actin (in green) did not seem to work on my human FFPE brain tissue. Appart from being really autofluorescent on the tissue, it seems to have a puncta-like staining on some cells, not the usual/expected clear cytoeskeleton staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
Beta Actin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:60008-1-Ig)
FH

P (Verified Customer) (09-23-2024)

Excellent

  • Applications: Western Blot
  • Primary Antibody Dilution: 2000
  • Cell Tissue Type: HEK
Beta Actin Antibody Western Blot validation (2000 dilution) in HEK (Cat no:60008-1-Ig)
FH

Udesh (Verified Customer) (08-16-2023)

The Ab worked well for both WB and IF at mentioned dilutions

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
  • Cell Tissue Type: Glioblastoma cells
FH

Chun (Verified Customer) (09-07-2020)

This is an excellent antibody for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HEK293, HeLa, HaCaT and SH-SY5Y cells
FH

Nikhil (Verified Customer) (09-17-2019)

Very good and specific. I can use 1:2500 5 ml antibody solution two times.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: Colon cancer cells
Beta Actin Antibody Western Blot, validation (1:2500 dilution) in Colon cancer cells (Cat no:60008-1-Ig)
FH

Leonardo (Verified Customer) (09-12-2019)

Excellent antibody. Works well since the first time.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: Human breast cancer (MDA-MB-231)
Beta Actin Antibody Western Blot, validation (1:5,000 dilution) in Human breast cancer (MDA-MB-231) (Cat no:60008-1-Ig)
FH

mark (Verified Customer) (02-05-2019)

Antibody works beautifully to detect a single Beta-actin band as a control

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human cancer cells
Beta Actin Antibody Western Blot, validation (1:2000 dilution) in human cancer cells (Cat no:60008-1-Ig)