Alpha cardiac muscle actin specific Monoklonaler Antikörper

Alpha cardiac muscle actin specific Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

1F2B9

Kat-Nr. : CL594-66125

Synonyme

ACTC1-specific, ACTC1, Alpha actin, 1F2B9, ACTC

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  IF Staining of mouse heart using CL594-66125

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-66125 (ACTC1-specific antibody) at dilution of 1:100.

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  IF Staining of mouse heart using CL594-66125

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-66125 (ACTC1-specific antibody) at dilution of 1:100.

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  FC experiment of C2C12 using CL594-66125

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACTC1-specific (CL594-66125, Clone:1F2B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-66125 bindet in IF-P, FC (Intra) Alpha cardiac muscle actin specific und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha, cardiac muscle 1
• Berechnetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_005159
• Gene symbol: ACTC1
• Gene ID (NCBI): 70
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publikationen

Species	Application	Title
mouse	IF	J Am Heart Assoc; α Protein Kinase 3 Is Essential for Neonatal and Adult Cardiac Function; Authors - Wei Feng