ARP2 Polyklonaler Antikörper

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• KD/KO Validated

ARP2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 10922-1-AP

Synonyme

ACTR2, Actin-like protein 2, Actin-related protein 2

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  WB analysis using 10922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10922-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 10922-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ARP2 antibody (10922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 10922-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human ARP2 (10922-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mausnierengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

10922-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA ARP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARP2 fusion protein Ag1359
• Vollständiger Name: ARP2 actin-related protein 2 homolog (yeast)
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC014546
• Gene symbol: ARP2/ACTR2
• Gene ID (NCBI): 10097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
		Nat Commun; Synergistic IL-6 and IL-8 paracrine signalling pathway infers a strategy to inhibit tumour cell migration.; Authors - Hasini Jayatilaka
	WB	Elife; Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein; Authors - Congyan Zhang
human	WB	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
human	IF	Elife; mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex.; Authors - Madeline G Andrews