ACVR1B Rekombinanter Antikörper

ACVR1B Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230260E12

Kat-Nr. : 83026-5-RR

Synonyme

230260E12, activin A receptor, type IB, Activin receptor like kinase 4, Activin receptor type 1B, Activin receptor type-1B

---

• 

  IF Staining of NIH/3T3 using 83026-5-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ACVR1B antibody (83026-5-RR, Clone: 230260E12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HEK-293 using 83026-5-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ACVR1B (83026-5-RR, Clone:230260E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83026-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83026-5-RR against Human ACVR1B were performed. The affinity constant is 16.1 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83026-5-RR bindet in IF/ICC, FC (Intra), ELISA ACVR1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ACVR1B fusion protein Ag0129
• Vollständiger Name: activin A receptor, type IB
• Berechnetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC000254
• Gene symbol: ACVR1B
• Gene ID (NCBI): 91
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACVR1B (ALK4, activin A receptor type 1B) is a transforming growth factor-beta (TGF-beta) superfamily member (PMID: 37020544). A potential role for ACVR1B in regulating muscle mass is that it is part of the transforming growth factor b (TGFb) pathway regulating myostatin (PMID: 21063444).