ADAM12 Polyklonaler Antikörper

ADAM12 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26928-1-AP

Synonyme

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

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Galerie der Validierungsdaten

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  WB analysis using 26928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 26928-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26928-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26928-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 26928-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 26928-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A431-Zellen, A549-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26928-1-AP bindet in WB, IHC,ELISA ADAM12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM12 fusion protein Ag17474
• Vollständiger Name: ADAM metallopeptidase domain 12
• Berechnetes Molekulargewicht: 909 aa, 100 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC060804
• Gene symbol: ADAM12
• Gene ID (NCBI): 8038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90 kDa mature (processed form that lack the prodomain), 50-68 kDa degradation product and 27 kDa prodomain.