ADAM28 Polyklonaler Antikörper

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• KD/KO Validated

ADAM28 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 22234-1-AP

Synonyme

ADAM 28, ADAM28, eMDC II, eMDCII, MDC L, MDC Lm, MDC Ls, MDCL

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 22234-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 22234-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 22234-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22234-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 22234-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 22234-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 22234-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 22234-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 22234-1-AP

  IP Result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, BxPC-3-Zellen, Maus-Dünndarmgewebe, Maus-Kolongewebe, Mauslebergewebe, Mausmilzgewebe, PC-13-Zellen
• Erfolgreiche IP: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below

Produktinformation

22234-1-AP bindet in WB, IP, ELISA ADAM28 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM28 fusion protein Ag17586
• Vollständiger Name: ADAM metallopeptidase domain 28
• Berechnetes Molekulargewicht: 775 aa, 87 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC136478
• Gene symbol: ADAM28
• Gene ID (NCBI): 10863
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
human	WB	Cancer Lett; ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.; Authors - Jia-Min Zhang ; KO Validated
mouse	WB	Biol Reprod; ADGRG2 is dispensable for lumicrine signalling regulating epididymal initial segment differentiation and gene expression†; Authors - Daiji Kiyozumi
human	WB	World J Gastroenterol; PMID: 31602160; Authors - Li Wei ; KD Validated
mouse	WB	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen