ADAMDEC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ADAMDEC1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 17899-1-AP

Synonyme

EC:3.4.24.-, ADAM-like protein decysin-1, ADAMDEC 1, ADAM DEC1, A disintegrin and metalloproteinase domain-like protein decysin-1

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  WB analysis of MCF-7 using 17899-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 17899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 17899-1-AP (ADAMDEC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 17899-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 17899-1-AP (ADAMDEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

17899-1-AP bindet in WB, IHC, IF/ICC, ELISA ADAMDEC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAMDEC1 fusion protein Ag12265
• Vollständiger Name: ADAM-like, decysin 1
• Berechnetes Molekulargewicht: 470 aa, 53 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC074877
• Gene symbol: ADAMDEC1
• Gene ID (NCBI): 27299
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) was first identified as a unique member of the matrix metalloprotease (ADAM) family. ADAMs are increasingly recognized as playing an important role in gut homeostasis and bowel inflammation. ADAMDEC1 is abundantly, and almost exclusively, expressed in the gastrointestinal (GI) tract.

Publikationen

Species	Application	Title
human	IHC	Arch Toxicol; A transcriptome-wide association study integrating multi-omics bioinformatics and Mendelian randomization reveals the prognostic value of ADAMDEC1 in colon cancer; Authors - Cong Zhang
human	IHC	J Inflamm Res; The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome; Authors - Haishi Zheng
human,mouse	WB,IHC	Thorac Cancer; Upregulation of ADAMDEC1 correlates with tumor progression and predicts poor prognosis in non-small cell lung cancer (NSCLC) via the PI3K/AKT pathway.; Authors - Weiliang Zhu
human	IHC	Aging (Albany NY); An immune signature to predict the prognosis of ATRX-wildtype glioma patients and guide immune checkpoint blockade therapy; Authors - Wenpeng Cao
human	WB	Sci Rep; ADAMDEC1 promotes the malignant progression of cholangiocarcinoma by regulating NF-κB signaling pathway; Authors - Shuo Zhou
human	WB,IHC	Exp Cell Res; ADAMDEC1 induces EMT and promotes colorectal cancer cells metastasis by enhancing Wnt/β-catenin signaling via negative modulation of GSK3β; Authors - Yuna Jia ; KD Validated