ADH1B Polyklonaler Antikörper

ADH1B Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 17165-1-AP

Synonyme

ADH1B, ADH2, Alcohol dehydrogenase 1B

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 17165-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17165-1-AP (ADH1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17165-1-AP

  IP result of anti-ADH1B (IP:17165-1-AP, 4ug; Detection:17165-1-AP 1:500) with mouse liver tissue lysate 4000 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

17165-1-AP bindet in WB, IP, IHC, IF, ELISA ADH1B und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADH1B fusion protein Ag10630
• Vollständiger Name: alcohol dehydrogenase 1B (class I), beta polypeptide
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC033009
• Gene symbol: ADH1B
• Gene ID (NCBI): 125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Publikationen

Species	Application	Title
	IF	Cell Death Discov; Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.; Authors - Lulin Zhou
	IF	Endocrinology; De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.; Authors - Tomoko Kawai
rat	WB	Arch Biochem Biophys; Baicalin ameliorates alcohol-induced hepatic steatosis by suppressing SREBP1c elicited PNPLA3 competitive binding to ATGL.; Authors - Pan Li
	IHC	Exp Eye Res; Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.; Authors - Jie Yang
rat	WB	J Sci Food Agric; Probiotic-fermented Pueraria lobata (Willd.) Ohwi alleviates alcoholic liver injury by enhancing antioxidant defense and modulating gut microbiota; Authors - Wenjun Zhao
	WB	Front Pharmacol; Identification of afatinib-associated ADH1B and potential small-molecule drugs targeting ADH1B for hepatocellular carcinoma; Authors - Yongxu Zhou