ADH1B Monoklonaler Antikörper

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• KD/KO Validated

ADH1B Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1F1B4

Kat-Nr. : 66939-1-Ig

Synonyme

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

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  WB analysis using 66939-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66939-1-Ig

  WB result of ADH1B antibody (66939-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH1B transfected HepG2 cells.

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  WB analysis of pig liver using 66939-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 66939-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66939-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66939-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66939-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1B antibody (66939-1-Ig, Clone: 1F1B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HepG2-Zellen, HuH-7-Zellen, Hausschwein-Lebergewebe, Rattenlebergewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66939-1-Ig bindet in WB, IHC, IF/ICC, ELISA ADH1B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADH1B fusion protein Ag10630
• Vollständiger Name: alcohol dehydrogenase 1B (class I), beta polypeptide
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC033009
• Gene symbol: ADH1B
• Gene ID (NCBI): 125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Publikationen

Species	Application	Title
human,mouse	WB,IHC	iScience; A positive feed-forward loop between Fusobacterium nucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.; Authors - Chi-Yao Hsueh
mouse	WB	Front Pharmacol; Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy.; Authors - Limin Gao
human	WB	Front Genet; Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing; Authors - Chengmao Xie
human	IHC	Cell Rep Methods; Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids; Authors - Guoyun Bi
human	IF	Oncoimmunology; HIF2A mediates lineage transition to aggressive phenotype of cancer-associated fibroblasts in lung cancer brain metastasis; Authors - Muyuan You
human	IF	Cancer Commun (Lond); Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma; Authors - Peiyu Wang