ADH4 Polyklonaler Antikörper

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• KD/KO Validated

ADH4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16474-1-AP

Synonyme

ADH 2, ADH4, Alcohol dehydrogenase 4

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  WB analysis of HepG2 using 16474-1-AP

  WB result of ADH4 antibody (16474-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH4 transfected HepG2 cells.

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  WB analysis using 16474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16474-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16474-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Maushirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

16474-1-AP bindet in WB, IHC, ELISA ADH4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADH4 fusion protein Ag9568
• Vollständiger Name: alcohol dehydrogenase 4 (class II), pi polypeptide
• Berechnetes Molekulargewicht: 380 aa, 40 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC022319
• Gene symbol: ADH4
• Gene ID (NCBI): 127
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
human	WB	Drug Metab Dispos; Characterization of human alcohol dehydrogenase 4 and aldehyde dehydrogenase 2 as enzymes involved in the formation of 5-carboxylpirfenidone, a major metabolite of pirfenidone; Authors - Rei Sato
Human		iScience; Crosstalk of non-apoptotic RCD panel in hepatocellular carcinoma reveals the prognostic and therapeutic optimization; Authors - Shuo Li
human	IHC	BMC Cancer; ADH4-a potential prognostic marker for hepatocellular carcinoma with possible immune-related implications; Authors - Ling Li