ADHFE1 Polyklonaler Antikörper

ADHFE1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14674-1-AP

Synonyme

ADH8, ADHFE1, HMFT2263, HOT

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Galerie der Validierungsdaten

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  WB analysis of rat kidney using 14674-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14674-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14674-1-AP (ADHFE1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14674-1-AP (ADHFE1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

14674-1-AP bindet in WB, IHC,ELISA ADHFE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADHFE1 fusion protein Ag6382
• Vollständiger Name: alcohol dehydrogenase, iron containing, 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 45 kDa
• GenBank-Zugangsnummer: BC064634
• Gene symbol: ADHFE1
• Gene ID (NCBI): 137872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.

Publikationen

Species	Application	Title
		Circ Cardiovasc Genet; Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease.; Authors - Holger Husi