ADI1 Polyklonaler Antikörper

ADI1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15133-1-AP

Synonyme

Aci reductone dioxygenase, acireductone dioxygenase 1, ADI1, APL1, ARD, HMFT1638, MTCBP 1, MTCBP1, SIP L, SIPL

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Galerie der Validierungsdaten

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  WB analysis of human testis using 15133-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15133-1-AP (ADI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 15133-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 15133-1-AP (ADI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15133-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15133-1-AP (ADI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15133-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15133-1-AP (ADI1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15133-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15133-1-AP (ADI1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

15133-1-AP bindet in WB, IHC,ELISA ADI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADI1 fusion protein Ag7274
• Vollständiger Name: acireductone dioxygenase 1
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC001467
• Gene symbol: ADI1
• Gene ID (NCBI): 55256
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADI1(acireductone dioxygenase 1 ) is also named as SIPL, ARD, APL1, HMFT1638, MTCBP-1, FLJ10913, MTCBP1 and belongs to the acireductone dioxygenase (ARD) family. It catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).The downregulation of ADI1 protein expression in human prostate cancer specimens argues that ADI1 potentially inhibits the growth that occurs during prostate cancer progression(PMID:17786183). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	IHC	J Cell Biol; Pancreatic tumor cell metastasis is restricted by MT1-MMP binding protein MTCBP-1.; Authors - Li Qiang
human	WB	Oncotarget; Arginine deiminase expressed in vivo, driven by human telomerase reverse transcriptase promoter, displays high hepatoma targeting and oncolytic efficiency.; Authors - Hui Jiang
human	WB	Biochem Biophys Res Commun; Clusterin and Pycr1 alterations associate with strain and model differences in susceptibility to experimental pancreatitis.; Authors - Sapna Iyer