AFP Polyklonaler Antikörper

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• KD/KO Validated

AFP Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(134)

Kat-Nr. : 14550-1-AP

Synonyme

HPAFP, Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein

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  WB analysis using 14550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14550-1-AP

  WB result of AFP antibody (14550-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells.

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  WB analysis of BxPC-3 using 14550-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14550-1-AP

  IP result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human ovary cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14550-1-AP (AFP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HepG2-Zellen, HuH-7-Zellen, humanes Plazenta-Gewebe, L02-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 36 publications below
• IHC: See 50 publications below
• IF: See 54 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14550-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA AFP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AFP fusion protein Ag6089
• Vollständiger Name: alpha-fetoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC027881
• Gene symbol: AFP
• Gene ID (NCBI): 174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

Publikationen

Species	Application	Title
mouse	IHC	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	IHC	Cancer Cell; Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.; Authors - Ruidong Xue
mouse	IF	Nat Commun; ATAXIN-2 intermediate-length polyglutamine expansions elicit ALS-associated metabolic and immune phenotypes; Authors - Renata Vieira de Sá
mouse	IHC	J Clin Invest; Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.; Authors - Xueru Mu
human	IHC	J Clin Invest; HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis.; Authors - Celine Hernandez
human,mouse	WB,IHC,IF	Nat Commun; Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.; Authors - Wei Cheng