AFP Monoklonaler Antikörper

AFP Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

4B3E4

Kat-Nr. : 67852-1-Ig

Synonyme

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

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Galerie der Validierungsdaten

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  WB analysis of HuH-7 using 67852-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67852-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67852-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67852-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67852-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67852-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HuH-7-Zellen, humanes Hodengewebe, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67852-1-Ig bindet in WB, ELISA AFP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AFP fusion protein Ag6089
• Vollständiger Name: alpha-fetoprotein
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC027881
• Gene symbol: AFP
• Gene ID (NCBI): 174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).