AGO2 Monoklonaler Antikörper

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• KD/KO Validated

AGO2 Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(64)

CloneNo.

1G2H12

Kat-Nr. : 67934-1-Ig

Synonyme

EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2

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  WB analysis using 67934-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 67934-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HepG2 using 67934-1-Ig

  WB result of AGO2 antibody (67934-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGO2 transfected HepG2 cells.

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  WB analysis of 4T1 using 67934-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 67934-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67934-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 67934-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug AGO2 Monoclonal antibody (67934-1-Ig, Clone:1G2H12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 5 publications below
• RIP: See 45 publications below

Produktinformation

67934-1-Ig bindet in WB, IHC, IF, FC (Intra), IP, RIP, ELISA AGO2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AGO2 fusion protein Ag27772
• Vollständiger Name: eukaryotic translation initiation factor 2C, 2
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC007633
• Gene symbol: EIF2C2
• Gene ID (NCBI): 27161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
mouse	RIP	Environ Health Perspect; Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study.; Authors - Bingwei Yang
human	WB,IP	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
mouse	RIP	Life Sci; LncRNA PEG11as aggravates cerebral ischemia/reperfusion injury after ischemic stroke through miR-342-5p/PFN1 axis; Authors - Zhijun Yu
mouse	WB	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
		Cancer Cell Int; LncRNA LZTS1-AS1 induces proliferation, metastasis and inhibits autophagy of pancreatic cancer cells through the miR-532 /TWIST1 signaling pathway; Authors - Hui Wu