GPAT3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GPAT3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 20603-1-AP

Synonyme

AGPAT9, 1-AGPAT 9, 1-AGP acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10

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  WB analysis of rat kidney using 20603-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20603-1-AP (AGPAT9 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20603-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20603-1-AP (AGPAT9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 20603-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20603-1-AP (AGPAT9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20603-1-AP (AGPAT9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20603-1-AP (AGPAT9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20603-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20603-1-AP (AGPAT9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20603-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AGPAT9 antibody (20603-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 20603-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AGPAT9 (20603-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, HeLa-Zellen, HT-1080.Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IF: See 1 publications below

Produktinformation

20603-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA GPAT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPAT3 fusion protein Ag14579
• Vollständiger Name: 1-acylglycerol-3-phosphate O-acyltransferase 9
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC006236
• Gene symbol: AGPAT9
• Gene ID (NCBI): 84803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.

Publikationen

Species	Application	Title
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Hum Mol Genet; PMID: 31873720; Authors - Mingming Gao ; KO Validated
mouse	WB,IF	Cell Death Dis; GPAT3 regulates the synthesis of lipid intermediate LPA and exacerbates Kupffer cell inflammation mediated by the ERK signaling pathway; Authors - Guoqiang Fan
human	WB	Ecotoxicol Environ Saf; Perturbation of arachidonic acid and glycerolipid metabolism promoted particulate matter-induced inflammatory responses in human bronchial epithelial cells; Authors - Jian Wang ; KD Validated
mouse	WB	Biomed Pharmacother; Si-Ni-San reverses dietary fat absorption defects in a murine model of depression; Authors - Yuanyuan Liu