CCP1 Polyklonaler Antikörper

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• KD/KO Validated

CCP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 14067-1-AP

Synonyme

AGTPBP1, ATP/GTP binding protein 1, CCP1, Cytosolic carboxypeptidase 1, KIAA1035, NNA1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14067-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14067-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14067-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14067-1-AP

  IP Result of anti-CCP1 (IP:14067-1-AP, 3ug; Detection:14067-1-AP 1:1000) with mouse testis tissue lysate 10000ug.

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  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14067-1-AP (CCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 14067-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 14067-1-AP (CCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Herzgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

14067-1-AP bindet in WB, IP, IHC, IF, ELISA CCP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCP1 fusion protein Ag5122
• Vollständiger Name: ATP/GTP binding protein 1
• Berechnetes Molekulargewicht: 138 kDa
• Beobachtetes Molekulargewicht: 138 kDa
• GenBank-Zugangsnummer: BC060815
• Gene symbol: AGTPBP1
• Gene ID (NCBI): 23287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.

Publikationen

Species	Application	Title
mouse	IF	Cell; Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis.; Authors - Carla G Silva
mouse	WB	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye ; KO Validated
human,mouse	WB,IF	EMBO J; Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.; Authors - Vandana Shashi
mouse	WB	Nat Commun; AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.; Authors - Albert Herms
rat	WB	Proc Natl Acad Sci U S A; Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.; Authors - Maria Elena Pero ; KO Validated
human,mouse	WB,IP	J Cell Biol; CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice.; Authors - Stephen Gilmore-Hall