Fetuin-A/AHSG Monoklonaler Antikörper

Fetuin-A/AHSG Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1F6B9

Kat-Nr. : 66094-1-Ig

Synonyme

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

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  WB analysis of HepG2 using 66094-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 66094-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66094-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 66094-1-Ig

  IP result of anti-Fetuin-A (IP:66094-1-Ig, 3ug; Detection:66094-1-Ig 1:3000) with HepG2 cells lysate 6000ug.

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  IHC staining of human liver cancer using 66094-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 66094-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 66094-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66094-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 66094-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Fetuin-A antibody (66094-1-Ig, Clone: 1F6B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

• 

  FC experiment of HepG2 using 66094-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig, Clone:1F6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Herzgewebe, humanes Plasmagewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

66094-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Fetuin-A/AHSG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fetuin-A/AHSG fusion protein Ag10073
• Vollständiger Name: alpha-2-HS-glycoprotein
• Berechnetes Molekulargewicht: 367 aa, 39 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC048198
• Gene symbol: Fetuin-A
• Gene ID (NCBI): 197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

Publikationen

Species	Application	Title
human	IF	Nature; Fetal hepatocytes protect the HSPC genome via fetuin-A; Authors - Xiao-Lin Guo