AIFM2/ FSP1 Rekombinanter Antikörper

AIFM2/ FSP1 Rekombinant Antikörper für WB, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

2J1

Kat-Nr. : 82974-1-RR

Synonyme

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

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  WB analysis using 82974-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82974-1-RR (AIFM2/ FSP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 82974-1-RR

  IP result of anti-AIFM2/ FSP1 (IP:82974-1-RR, 4ug; Detection:82974-1-RR 1:3000) with HepG2 cells lysate 1085 ug.

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  FC experiment of A549 using 82974-1-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug AIFM2/ FSP1 Recombinant antibody (82974-1-RR, Clone:2J1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82974-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82974-1-RR against Human AIFM2/ FSP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82974-1-RR bindet in WB, FC (Intra), IP, ELISA AIFM2/ FSP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AIFM2/ FSP1 fusion protein Ag13412
• Vollständiger Name: apoptosis-inducing factor, mitochondrion-associated, 2
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC023601
• Gene symbol: AIFM2/ FSP1
• Gene ID (NCBI): 84883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.