AIP/ARA9 Polyklonaler Antikörper

AIP/ARA9 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 18176-1-AP

Synonyme

AIP, ARA9, XAP 2, XAP2, AH receptor-interacting protein

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  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18176-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18176-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18176-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18176-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 18176-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug AIP/ARA9 Polyclonal antibody (18176-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

18176-1-AP bindet in WB, IHC, FC (Intra), IP, ELISA AIP/ARA9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIP/ARA9 fusion protein Ag12869
• Vollständiger Name: aryl hydrocarbon receptor interacting protein
• Berechnetes Molekulargewicht: 330 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC104797
• Gene symbol: AIP
• Gene ID (NCBI): 9049
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IP	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk
rat	WB,IHC	Heliyon; PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats; Authors - Wei Zhang