AIRE Polyklonaler Antikörper

AIRE Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 22517-1-AP

Synonyme

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1

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Galerie der Validierungsdaten

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  WB analysis of L02 using 22517-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 22517-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 22517-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 22517-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 22517-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 22517-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22517-1-AP (AIRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thymus using 22517-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22517-1-AP (AIRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22517-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 22517-1-AP (AIRE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, humanes Milzgewebe, humanes Plasmagewebe, Maus-Thymusgewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

22517-1-AP bindet in WB, IHC, IF, ELISA AIRE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AIRE fusion protein Ag18249
• Vollständiger Name: autoimmune regulator
• Berechnetes Molekulargewicht: 545 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58-69 kDa
• GenBank-Zugangsnummer: BC137270
• Gene symbol: AIRE
• Gene ID (NCBI): 326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The function of the protein is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Publikationen

Species	Application	Title
mouse	WB	Nat Immunol; Chd4 choreographs self-antigen expression for central immune tolerance.; Authors - Yoshihiko Tomofuji
mouse	WB	Biochemistry (Mosc); Defective Central Immune Tolerance Induced by High-Dose D-Galactose Resembles Aging.; Authors - H M Du
human	IF	Nat Commun; The immune landscape of human thymic epithelial tumors; Authors - Zhongwei Xin