AKAP1 Polyklonaler Antikörper

AKAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15618-1-AP

Synonyme

D-AKAP-1, DAKAP1, A-kinase anchor protein 149 kDa, A-kinase anchor protein 1, mitochondrial, AKAP 1

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  WB analysis using 15618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15618-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 15618-1-AP

  IP result of anti-AKAP1 (IP:15618-1-AP, 4ug; Detection:15618-1-AP 1:2000) with PC-3 cells lysate 1800ug.

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  IHC staining of human lung cancer using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 15618-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 15618-1-AP

  Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Lebergewebe, MCF-7-Zellen, PC-3-Zellen, SW480-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

15618-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA AKAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKAP1 fusion protein Ag8037
• Vollständiger Name: A kinase (PRKA) anchor protein 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 149 kDa
• GenBank-Zugangsnummer: BC000729
• Gene symbol: AKAP1
• Gene ID (NCBI): 8165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Publikationen

Species	Application	Title
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu
human	WB,IP,IF	Mol Endocrinol; Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis.; Authors - Grozdanov Petar N PN
human	WB,RIP,IHC	Noncoding RNA Res; LINC00323 knockdown suppresses the proliferation, migration, and vascular mimicry of non-small cell lung cancer cells by promoting ubiquitinated degradation of AKAP1; Authors - Bin Ke ; KD Validated
human	WB	Cell Death Differ; AKAP1/PKA-mediated GRP75 phosphorylation at mitochondria-associated endoplasmic reticulum membranes protects cancer cells against ferroptosis; Authors - Hao Liu