ALDH1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ALDH1A1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

114

Rezensionen (6)

Kat-Nr. : 15910-1-AP

Synonyme

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 15910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15910-1-AP

  IP result of anti-ALDH1A1(IP:15910-1-AP, 4ug; Detection:15910-1-AP 1:10000) with mouse liver tissue lysate 3040 ug.

• 

  IHC staining of mouse testis using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse colon using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 15910-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15910-1-AP

  Immunofluorescent analysis of HepG2 cells using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15910-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (15910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushirngewebe, Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Kolongewebe, HepG2-Zellen, Maushodengewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 96 publications below
• IHC: See 21 publications below
• IF: See 13 publications below
• ELISA: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15910-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA ALDH1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1A1 fusion protein Ag8665
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A1
• Berechnetes Molekulargewicht: 501 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC001505
• Gene symbol: ALDH1A1
• Gene ID (NCBI): 216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.

Publikationen

Species	Application	Title
human	WB	Cell Res; Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.; Authors - Xingxing Xu
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human	WB,IHC,IF	Int J Oral Sci; LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma; Authors - Yuantong Liu
human	WB,IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
human	IF	EMBO J; RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis.; Authors - Maria Laura Tognoli
human	WB	Theranostics; miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.; Authors - Qing Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Shinford Z. (Verified Customer) (01-31-2024)

The antibody performs exceptionally well; the band on the membrane is remarkably distinct.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:15910-1-AP)

FH

Matthew (Verified Customer) (08-07-2023)

Antibody worked perfectly with overnight incubation at 4C. Diluted 1:1000 in TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse mammary epithelial cell

FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:15910-1-AP)

FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working good for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 WB;

FH

Mario Armando (Verified Customer) (02-18-2020)

I used this ALDH1A1 antibody on different human and mouse tissues including adipose tissue, heart and skeletal muscle. It worked wonderfully even when using paraffin embedded sections in both human and mouse.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: OCT embedded frozen section of Human Adipose tissue

ALDH1A1 Antibody Immunofluorescence, validation (1:100 dilution) in OCT embedded frozen section of Human Adipose tissue (Cat no:15910-1-AP)

FH

Joleen (Verified Customer) (06-07-2019)

Prominent band correspond to ALDH1A1. Works well in western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ALDH1A1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15910-1-AP)

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C

ADH1B Polyclonal antibody

17165-1-AP

ADH6 Polyclonal antibody

13288-2-AP

RDH10 Polyclonal antibody

14644-1-AP

ADH7 Polyclonal antibody

23425-1-AP

ADH4 Polyclonal antibody

16474-1-AP