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ALDH1A1 Monoklonaler Antikörper

ALDH1A1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

CloneNo.

1A10A2

Kat-Nr. : 60171-1-Ig

Synonyme

1A10A2, 3-deoxyglucosone dehydrogenase, ALDC, Aldehyde dehydrogenase 1A1, ALDH 1A1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HepG2-Zellen, HuH-7-Zellen, K-562-Zellen, Hausschwein-Lebergewebe, Rattenlebergewebe
Erfolgreiche Detektion in IHChumanes Hirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60171-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ALDH1A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ALDH1A1 fusion protein Ag8551
Vollständiger Name aldehyde dehydrogenase 1 family, member A1
Berechnetes Molekulargewicht 501 aa, 55 kDa
Beobachtetes Molekulargewicht 52 kDa
GenBank-ZugangsnummerBC001505
Gene symbol ALDH1A1
Gene ID (NCBI) 216
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ALDH1A1 antibody 60171-1-IgProtokoll herunterladen
IHC protocol for ALDH1A1 antibody 60171-1-IgProtokoll herunterladenl
IF protocol for ALDH1A1 antibody 60171-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
humanIF

Cell Rep Med

Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer

Authors - Cui-Cui Liu
mouseIF

Elife

Perirenal adipose tissue contains a subpopulation of cold-inducible adipocytes derived from brown-to-white conversion

Authors - Houyu Zhang
humanWB,CoIP

iScience

Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression

Authors - Zhilei Zhang
  • KD Validated
humanWB

Front Pharmacol

Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.

Authors - Ming Xin
humanWB

Oral Dis

microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1.

Authors - Kaixin Wangzhou