- Featured Product
- KD/KO Validated
ALDH1A1 Monoklonaler Antikörper
ALDH1A1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1A10A2
Kat-Nr. : 60171-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A549-Zellen, HepG2-Zellen, HuH-7-Zellen, K-562-Zellen, Hausschwein-Lebergewebe, Rattenlebergewebe |
Erfolgreiche Detektion in IHC | humanes Hirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 11 publications below |
IHC | See 2 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
60171-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ALDH1A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | ALDH1A1 fusion protein Ag8551 |
Vollständiger Name | aldehyde dehydrogenase 1 family, member A1 |
Berechnetes Molekulargewicht | 501 aa, 55 kDa |
Beobachtetes Molekulargewicht | 52 kDa |
GenBank-Zugangsnummer | BC001505 |
Gene symbol | ALDH1A1 |
Gene ID (NCBI) | 216 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for ALDH1A1 antibody 60171-1-Ig | Protokoll herunterladen |
IHC protocol for ALDH1A1 antibody 60171-1-Ig | Protokoll herunterladenl |
IF protocol for ALDH1A1 antibody 60171-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Cell Rep Med Targeting EMSY-mediated methionine metabolism is a potential therapeutic strategy for triple-negative breast cancer | ||
Elife Perirenal adipose tissue contains a subpopulation of cold-inducible adipocytes derived from brown-to-white conversion | ||
iScience Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression
| ||
Front Pharmacol Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells. | ||
Oral Dis microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1. |