ALDH1A2 Polyklonaler Antikörper

ALDH1A2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 13951-1-AP

Synonyme

ALDH1A2, RALDH 2, RALDH(II), RALDH2, RALDH2 T, Retinal dehydrogenase 2

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 13951-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13951-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13951-1-AP (ALDH1A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13951-1-AP

  IP Result of anti-ALDH1A2 (IP:13951-1-AP, 4ug; Detection:13951-1-AP 1:300) with mouse testis tissue lysate 4400ug.

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  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13951-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13951-1-AP (ALDH1A2 Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 5 publications below

Produktinformation

13951-1-AP bindet in WB, IP, IHC, ELISA ALDH1A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1A2 fusion protein Ag5062
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A2
• Berechnetes Molekulargewicht: 518 aa, 57 kDa
• Beobachtetes Molekulargewicht: 53-57 kDa
• GenBank-Zugangsnummer: BC030589
• Gene symbol: ALDH1A2
• Gene ID (NCBI): 8854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
human	WB	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang
human	WB	Am J Cancer Res; Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression; Authors - Bing-Yen Wang
human	IHC	J Lipid Res; Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.; Authors - Samuel L Arnold
mouse	WB	PLoS One; Retinoic acid signalling is activated in the postischemic heart and may influence remodelling.; Authors - Bilbija Dusan D