ALDH1B1 Polyklonaler Antikörper

ALDH1B1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 15560-1-AP

Synonyme

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5

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  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15560-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15560-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15560-1-AP (ALDH1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15560-1-AP

  IP result of anti-ALDH1B1 (IP:15560-1-AP, 4ug; Detection:15560-1-AP 1:20000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15560-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15560-1-AP (ALDH1B1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15560-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ALDH1B1 antibody (15560-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 8 publications below

Produktinformation

15560-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ALDH1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1B1 fusion protein Ag7144
• Vollständiger Name: aldehyde dehydrogenase 1 family, member B1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC001619
• Gene symbol: ALDH1B1
• Gene ID (NCBI): 219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.; Authors - Nina Oberbeck
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
mouse	IHC	J Transl Med; Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque; Authors - Fu-Jun Liao
mouse	WB,IHC	J Pathol; Effects of long-term ethanol consumption and Aldh1b1 depletion on intestinal tumourigenesis in mice.; Authors - Mike F Müller
mouse	WB	J Ethnopharmacol; Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice; Authors - Si-Wei Rao
human	WB	Chin Med; Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis; Authors - Jianhua Zhen