ALDH1L2 Polyklonaler Antikörper

ALDH1L2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 21391-1-AP

Synonyme

ALDH1L2

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Galerie der Validierungsdaten

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  WB analysis using 21391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IF Staining of A431 using 21391-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ALDH1L2 antibody (21391-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

21391-1-AP bindet in WB, IHC, IF, ELISA ALDH1L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH1L2 fusion protein Ag16046
• Vollständiger Name: aldehyde dehydrogenase 1 family, member L2
• Berechnetes Molekulargewicht: 923 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa, 89 kDa
• GenBank-Zugangsnummer: BC103935
• Gene symbol: ALDH1L2
• Gene ID (NCBI): 160428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1L2(Aldehyde dehydrogenase family 1 member L2) is also named as mitochondrial 10-FTHFDH, mtFDH and belongs to the aldehyde dehydrogenase family and ALDH1L subfamily. The ALDH1L2 gene has three transcriptional variants with the molecular weight of 102 kDa, 42 kDa and 89 kDa(PMID:19823103). It is a mitochondrial enzyme, a likely source of CO2 production from 10-formyltetrahydrofolate in mitochondria and playing an essential role in the distribution of one-carbon groups between the cytosolic and mitochondrial compartments of the cell(PMID: 20498374). It also has dehydrogenase/hydrolase activities similar to cytosolic FDH (ALDH1L1). The native ALDH1L2 has been reported to exist as a dimer or tetramer(PMID:7822273).

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
pig	WB	Mol Metab; Maternal hyperglycemia induces alterations in hepatic amino acid, glucose and lipid metabolism of neonatal offspring: Multi-omics insights from a diabetic pig model; Authors - Bachuki Shashikadze
human,mouse	WB,IHC	Free Radic Biol Med; Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.; Authors - Yunwen Yang
human	WB	J Biol Chem; Acetylation of aldehyde dehydrogenase ALDH1L2 regulates cellular redox balance and the chemosensitivity of colorectal cancer to 5-Fluorouracil; Authors - Chaoqun Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: liver, kidney, hippocampus

ALDH1L2 Antibody Western Blot, validation (1:1000 dilution) in liver, kidney, hippocampus (Cat no:21391-1-AP)