ALDH2 Monoklonaler Antikörper

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• KD/KO Validated

ALDH2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2G10F4

Kat-Nr. : 68237-1-Ig

Synonyme

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

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  WB analysis of mouse liver using 68237-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 68237-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 68237-1-Ig

  WB result of ALDH2 antibody (68237-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH2 transfected HepG2 cells.

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  WB analysis of rabbit liver using 68237-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 68237-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68237-1-Ig (ALDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Kaninchenlebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

68237-1-Ig bindet in WB, ELISA ALDH2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDH2 fusion protein Ag7463
• Vollständiger Name: aldehyde dehydrogenase 2 family (mitochondrial)
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC002967
• Gene symbol: ALDH2
• Gene ID (NCBI): 217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Publikationen

Species	Application	Title
mouse	WB	Arch Biochem Biophys; Inhibiting apoptosis and GSDME-mediated pyroptosis attenuates hepatic injury in septic mice; Authors - Na Lu
mouse	WB	Mol Nutr Food Res; Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice; Authors - Meng-Yuan Liu