ALDH3B1 Polyklonaler Antikörper

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• KD/KO Validated

ALDH3B1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 19446-1-AP

Synonyme

Aldehyde dehydrogenase 7, ALDH3B1, ALDH4, ALDH7

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 19446-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 19446-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 19446-1-AP (ALDH3B1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

19446-1-AP bindet in WB, IHC, ELISA ALDH3B1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH3B1 fusion protein Ag7025
• Vollständiger Name: aldehyde dehydrogenase 3 family, member B1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC002553
• Gene symbol: ALDH3B1
• Gene ID (NCBI): 221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH3B1(Aldehyde dehydrogenase family 3 member B1) is also named as ALDH7 and belongs to the aldehyde dehydrogenase family. It catalyzes fatty and aromatic aldehydes. ALDH3B1 may play a role in the detoxification of aldehydes produced during oxidative stress. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Elife; FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.; Authors - Carlos J Diaz Osterman
human	IHC	Cell Death Dis; Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression; Authors - Yue Wang
human	WB	Front Immunol; The ALDH Family Contributes to Immunocyte Infiltration, Proliferation and Epithelial-Mesenchymal Transformation in Glioma.; Authors - Zeyu Wang ; KD Validated
mouse	WB	Cell Stress Chaperones; ALDH3B1 protects interfollicular epidermal cells against lipid peroxidation via the NRF2 pathway; Authors - Zhenjie Wu