ALDOA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ALDOA Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

44

Rezensionen (1)

Kat-Nr. : 11217-1-AP

Synonyme

ALDA, ALDOA, Aldolase A, Lung cancer antigen NY LU 1, Muscle type aldolase

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11217-1-AP

  WB result of ALDOA antibody (11217-1-AP; 1:240000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HeLa cells.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11217-1-AP (ALDOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11217-1-AP

  IP Result of anti-ALDOA (IP:11217-1-AP, 3ug; Detection:11217-1-AP 1:1000) with HepG2 cells lysate 6000ug.

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11217-1-AP (ALDOA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11217-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11217-1-AP (ALDOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11217-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11217-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (11217-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11217-1-AP

  1X10^6 HeLa cells were stained with 0.2ug ALDOA antibody (11217-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 39 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11217-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA ALDOA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDOA fusion protein Ag1726
• Vollständiger Name: aldolase A, fructose-bisphosphate
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC016800
• Gene symbol: ALDOA
• Gene ID (NCBI): 226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
mouse	WB	Dev Cell; Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.; Authors - Vinay Bulusu
human,mouse	WB	Int J Biol Sci; Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway; Authors - Yunfeng Guan
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	eLife; Glycolytic flux-signaling controls mouse embryo mesoderm development; Authors - Hidenobu Miyazawa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

K (Verified Customer) (12-20-2020)

Worked well for IHC at 1:200 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse liver tissues and rat liver