ALDOA Rekombinanter Antikörper

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• KD/KO Validated

ALDOA Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4

Kat-Nr. : 80837-1-RR

Synonyme

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

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  WB analysis using 80837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80837-1-RR (ALDOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 80837-1-RR

  WB result of ALDOA antibody (80837-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HEK-293 cells.

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  IF Staining of HeLa using 80837-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 80837-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 80837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80837-1-RR against Human ALDOA were performed. The affinity constant is 1.34 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Hausschwein-Lebergewebe, Hausschwein-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80837-1-RR bindet in WB, IF/ICC, ELISA ALDOA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALDOA fusion protein Ag1726
• Vollständiger Name: aldolase A, fructose-bisphosphate
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC016800
• Gene symbol: ALDOA
• Gene ID (NCBI): 226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).