Aldolase C Polyklonaler Antikörper

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• KD/KO Validated

Aldolase C Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 14884-1-AP

Synonyme

ALDOC, Aldolase C, Brain type aldolase, Fructose bisphosphate aldolase C

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Galerie der Validierungsdaten

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  WB analysis using 14884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 14884-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14884-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14884-1-AP (Aldolase C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14884-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14884-1-AP (Aldolase C antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14884-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14884-1-AP (Aldolase C antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aldolase C antibody (14884-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Aldolase C antibody (14884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14884-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14884-1-AP (Aldolase C antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Herzgewebe, Maus-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

14884-1-AP bindet in WB, IHC, IF, ELISA Aldolase C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Aldolase C fusion protein Ag6659
• Vollständiger Name: aldolase C, fructose-bisphosphate
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC003613
• Gene symbol: ALDOC
• Gene ID (NCBI): 230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involved in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).14884-1-AP can recognize Aldolase A and Aldolase C

Publikationen

Species	Application	Title
human	WB	Nat Commun; O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.; Authors - Kibum Kim
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	IF	Hum Mol Genet; Lack of CUL4B leads to increased abundance of GFAP-positive cells that is mediated by PTGDS in mouse brain.; Authors - Wei Zhao
human	WB	Stem Cell Reports; The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.; Authors - Zihao Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kazu (Verified Customer) (11-22-2022)

This antibody worked on frozen mouse brain tissue sections. A good astrocyte marker.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse brain