ALG5 Polyklonaler Antikörper

ALG5 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16046-1-AP

Synonyme

HSPC149, EC:2.4.1.117, DolP-glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog

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  WB analysis of human kidney using 16046-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16046-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 16046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 16046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 16046-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16046-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 16046-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16046-1-AP bindet in WB, IHC, IF/ICC, ELISA ALG5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALG5 fusion protein Ag8990
• Vollständiger Name: asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 324 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC012531
• Gene symbol: ALG5
• Gene ID (NCBI): 29880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Mol Life Sci; ALG5 downregulation inhibits osteogenesis and promotes adipogenesis by regulating the N-glycosylation of SLC6A9 in osteoporosis; Authors - Quanfeng Li ; KD Validated