ALK Monoklonaler Antikörper

ALK Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

7G9E3

Kat-Nr. : 60321-1-Ig

Synonyme

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK

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Galerie der Validierungsdaten

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  IHC staining of human lymphoma using 60321-1-Ig

  Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma (ALCL) tissue slide using 60321-1-Ig (ALK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 60321-1-Ig

  Immunohistochemical analysis of paraffin-embedded human anaplastic large cell lymphoma (ALCL) tissue slide using 60321-1-Ig (ALK/CD246 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

60321-1-Ig bindet in WB, IHC, ELISA ALK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALK fusion protein Ag21493
• Vollständiger Name: anaplastic lymphoma receptor tyrosine kinase
• Berechnetes Molekulargewicht: 1620 aa, 176 kDa
• GenBank-Zugangsnummer: NM_004304
• Gene symbol: ALK
• Gene ID (NCBI): 238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).

Publikationen

Species	Application	Title
human	WB	Eur J Med Chem; Discovery of 2,4-pyrimidinediamine derivatives as potent dual inhibitors of ALK and HDAC.; Authors - Tao Pan
human	WB	Neoplasma; miR-1271 inhibits OSCC cell growth and metastasis by targeting ALK.; Authors - D Kong
human	WB	iScience; DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer; Authors - Qingwen Huang

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