AMPD1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMPD1-Specific Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 19780-1-AP

Synonyme

AMP deaminase 1, AMP deaminase isoform M, AMPD1, AMPD1-Specific, MAD, MADA, Myoadenylate deaminase

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  WB analysis of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 19780-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:300.

• 

  IHC staining of mouse skeletal muscle using 19780-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19780-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19780-1-AP (AMPD1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

19780-1-AP bindet in WB, IHC, IP, ELISA AMPD1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: adenosine monophosphate deaminase 1 (isoform M)
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 75-83 kDa
• GenBank-Zugangsnummer: NM_000036
• Gene symbol: AMPD1
• Gene ID (NCBI): 270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMPD1, also named as MAD and MADA, belongs to the adenosine and AMP deaminases family. AMP deaminase plays a critical role in energy metabolism. It catalyzes the reaction: AMP + H2O = IMP + NH3. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM) which is a metabolic disorder resulting in exercise-related myopathy. The antibody is specific to AMPD1.

Publikationen

Species	Application	Title
mouse	WB	iScience; Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease; Authors - Ana Andres-Hernando ; KO Validated
human	IHC	Front Oncol; AMPD1 Is Associated With the Immune Response and Serves as a Prognostic Marker in HER2-Positive Breast Cancer.; Authors - Long Wang
mouse	WB	Sci Adv; Lactate metabolism is essential in early-onset mitochondrial myopathy; Authors - Zhenkang Chen
human	IHC	J Cell Mol Med; EDNRA regulates the tumour immune environment and predicts the efficacy and prognosis of cancer immunotherapy; Authors - Mengxue Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laila (Verified Customer) (07-22-2022)

This antibody worked great with Bovine tissue with a total of 60 ug of protein and very little non-specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: bovine skeletal muscle

AMPD1-Specific Antibody Western Blot validation (1:1000 dilution) in bovine skeletal muscle (Cat no:19780-1-AP)