AMPD3 Polyklonaler Antikörper

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• KD/KO Validated

AMPD3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 23997-1-AP

Synonyme

AMPD 3, EC:3.5.4.6

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  WB analysis using 23997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 23997-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 23997-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 23997-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23997-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23997-1-AP (AMPD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 23997-1-AP

  IP result of anti-AMPD3 (IP:23997-1-AP, 4ug; Detection:23997-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23997-1-AP (AMPD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 23997-1-AP (AMPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 23997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23997-1-AP (AMPD3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, HEK-293-Zellen, Mausnierengewebe, Mauslungengewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

23997-1-AP bindet in WB, IHC, IP, CoIP, ELISA AMPD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPD3 fusion protein Ag21199
• Vollständiger Name: adenosine monophosphate deaminase (isoform E)
• Berechnetes Molekulargewicht: 776 aa, 90 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 90 kDa
• GenBank-Zugangsnummer: BC126118
• Gene symbol: AMPD3
• Gene ID (NCBI): 272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IP	J Mol Cell Cardiol; Translational regulation by miR-301b upregulates AMP deaminase in diabetic hearts.; Authors - Yuki Tatekoshi
		Mol Cell Biochem; Role of AMP deaminase in diabetic cardiomyopathy; Authors - Tetsuji Miura
rat	WB	J Mol Cell Cardiol; Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload.; Authors - Yusuke Igaki
mouse,human	WB,IHC	Nat Metab; Super-enhancer-driven metabolic reprogramming promotes cystogenesis in autosomal dominant polycystic kidney disease.; Authors - Zeyun Mi ; KD Validated
mouse	WB	J Med Chem; Discovery of Novel N-(5-(Pyridin-3-yl)-1H-indazol-3-yl)benzamide Derivatives as Potent Cyclin-Dependent Kinase 7 Inhibitors for the Treatment of Autosomal Dominant Polycystic Kidney Disease; Authors - Bowen Yang
rat	WB,IP	Physiol Rep; Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts; Authors - Toshifumi Ogawa